MOLECULAR GENETIC MARKERS A MseI RFLP at the goat as2-casein gene

Source/description: A polymorphic MseI restriction site has been observed in a 1.3-kb DNA fragment of the goat as2-casein gene. PCR primers, TL30 and TQ9, were designed from regions of goat as2-Cn cDNA sequence (GenBank accession number X65160) corresponding to exons 5 and 7 of bovine as2-Cn gene (GenBank accession number M943227). Two alleles have been found: the A allele (showing a fragment of about 300 bp); and the B allele (showing a fragment of about 400 bp) (Fig. 1).